Leaf tissue analysis should also be used to diagnose or confirm nutrient deficiencies, particularly where visual symptoms are confusing, not visible, or where multiple nutrient deficiencies occur. It is important to ensure that tissue analysis follows standard procedures. There are two commonly accepted procedures adopted in different producing countries. Both analyze the middle lamina or inner part of the blade of leaf number 3 – the third fully expanded leaf on the plant at flowering or shooting (see photo below).
The most widely practiced method (IRS, International Reference System) analyzes the inner leaf area, but it is also acceptable to assess the nutrient status of the whole transversal strip.
It is important to be aware of which of these methods has been used as the accepted optimum leaf nutrient standards vary according to the analysis (Table 4) and the exact section in the leaf strip that is tested.